Axl Imperial Ltd. and ThetaMetrisis have signed an agreement for the distribution of ThetaMetrisis products in the industrial markets of Greece and Cyprus. Prospective customers from this area are kindly requested to contact the Axl Imperal Ltd sales team for further information on ThetaMetrisis products and demonstration of their capabilities.

Mr. George Rakanides

Tel: +30 210 725 7712

Website: https://axlimperial.com/

Biosensing through White Light Reflectance Spectroscopy (WLRS) is based on monitoring the shift of interference spectrum due to the binding reactions occurring on top of a thin SiO2 layer deposited on a silicon chip. Multianalyte determinations were possible through scanning of a single sensor chip on which multiple bioreactive areas have been created. Nonetheless, the implementation of moving parts increased the instrumentation size and complexity and limited the potential for on-site determinations. Thus, in this work, a new approach, which is based on patterning the sensor surface to create areas with different SiO2 thickness, is developed and evaluated for multi-analyte determinations with the WLRS set-up. The areas of different thickness can be interrogated by a single reflection probe placed on a fixed position over the chip and the reflection spectrum recorded is deconvoluted to the spectra corresponding to each area allowing the simultaneous monitoring of the bioreactions taking place at each one of them. The combination of different areas thickness was optimized using chips with two areas for single analyte assays. The optimum chips were then used for the simultaneous determination of two mycotoxins, aflatoxin B1 and fumonisin B1. A competitive immunoassay format was followed employing immobilization of mycotoxin-protein conjugates onto the SiO2 of different thickness. It was found that the dual-analyte assays had identical analytical characteristics with the respective single-analyte ones. The detection limits achieved were 0.05 ng/mL for aflatoxin B1 and 1.0 ng/mL for fumonisin B1, with dynamic ranges extending up to 5.0 and 50 ng/mL, respectively. The sensor was also evaluated for the determination of the two mycotoxins in whole grain samples (wheat and maize). The extraction protocol was optimized and recoveries ranging from 85 to 115% have been determined. Due to lack of moving parts, the novel multi-analyte format is expected to considerably facilitate the built-up of a portable device for determination of analytes at the point-of-need.

V. Anastasiadis, G. Koukouvinos, P.S. Petrou, A. Economou, M. Harjanne, P. Heimala, D. Goustouridis, I. Raptis, S.E. Kakabakos Biosens. Bioelectron. 153 112035(2020) “Multiplexed mycotoxins determination employing white light reflectance spectroscopy and silicon chips with silicon oxide areas of different thickness”

I & A Technology Inc. and ThetaMetrisis have signed an agreement for the distribution of ThetaMetrisis products in the market of South Korea. Prospective customers from this area are kindly requested to contact the I & A Technology sales team for further information on ThetaMetrisis products and demonstration of their capabilities.

Tel: +82-31-203-7978

Website: http://ina-technology.com/

MICL Consulting & Logistics and ThetaMetrisis has signed an agreement for the distribution of ThetaMetrisis products in the market of Israel. Prospective customers from Israel are kindly requested to contact the MICL sales team for further information on ThetaMetrisis products and demonstration of their capabilities.

Tel: +972-4-8343966

Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Website: https://micl-group.com/

 

Picotronica and ThetaMetrisis has signed an agreement for the distribution of ThetaMetrisis products in the market of Africa. Prospective customers from Africa are kindly requested to contact Dr Mohammad Mahmoud for further information on ThetaMetrisis products and demonstration of their capabilities.

Tel: +20 2 2235 6324

Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Website: http://www.picotronica.com

A miniaturized optical immunosensor based on White Light Reflectance Spectroscopy (WLRS) for the rapid and label-free detection of aflatoxin M1 (AFM1) in milk samples has been developed. WLRS sensing system consists of the measurement set-up and the biochip. The first encompasses the reflection probe, the light source, and the spectrometer, while the latter is a Si chip with a SiO2 layer on top where an AFM1-bovine serum albumin conjugate has been immobilized. The assay was performed by running mixtures of rabbit polyclonal anti-AFM1 antibody with the calibrators or the samples, followed by reaction with biotinylated anti-rabbit IgG antibody and streptavidin. The assay cycle was completed in 25 min, the limit of detection was 6 pg/mL, and the linear working range extended from 0.012 to 2.0 ng/mL. The assay was repeatable (intra-and inter-assay coefficients of variation ranged from 2.1 to 6.3% and 3.5 to 8.2%, respectively) and accurate (percent recovery values ranged from 92.5 to 110%). AFM1 could be detected with the immunosensor developed in both processed and unprocessed milk of different animal species without any dilution. The excellent analytical characteristics and the small instrument size make the proposed sensor suitable for accurate low-cost AFM1 determination in milk samples at the point-of-need.

D.Tsounidi, G. Koukouvinos, P. Petrou, K. Misiakos, G. Zisis, D. Goustouridis, I. Raptis, S. E. Kakabakos Sens. Act. B 282 104(2019) “Rapid and sensitive label-free determination of aflatoxin M1 levels in milk through a White Light Reflectance Spectroscopy immunosensor”

 

We are excited to announce that we are moving to a new location on April 1st. The move was driven by a requirement for more space in all departments, that will enable us to manufacture efficiently a larger quantity of products, and accommodate larger machines to support our growing client base.

Our new address is
Christou Lada 40
12132 Athens, Greece

Please use this address for your shipments.

Our telephone numbers (+30-213-0373544, +30-2130373547) remain the same.
 

 

A new product, FR-uProbe is released. FR-uProbe is a module for reflectance & trasnmittance measurements through any trinocular optical microscope with C-mount.

FR-uProbe is the solution for coating characterization applications that require spot size as small as very few micrometers, such as micropatterned surfaces, samples with irregular surfaces that exhibit a high level of scattering light and numerous others.

With FR-uProbe, local film thickness, optical constants, reflectance, transmission, and absorbance measurements across UV/Vis/NIR, is just a matter of a click.

The standard spot size (area from which the reflectactance or transmittance signal is collected) for 50X objective lens is 5μm and can be as small as 2μm.

FR-uProbe on an optical microscope

 For more information please contact our sales team at: This email address is being protected from spambots. You need JavaScript enabled to view it.

The fast and accurate determination of C-reactive protein (CRP) in human whole blood samples employing a WLRS sensing platform is demonstrated. Each assay cycle was completed in 12 min, including 5-min reaction with the diluted whole blood samples, 3-min with the biotinylated anti-CRP antibody, and 4-min with streptavidin solution. The effect of whole blood on the assay performance was evaluated, and it was found that dilutions as low as 50-times could be employed. Thus, taking into account that the assay had a detection limit of 2.2 ug/L CRP in 50-times diluted sample, whole blood concentrations as low as 110 ug/L could be determined. The excellent analytical characteristics, the short analysis time and the compact instrument size support the applicability of the immunosensor developed for point-of-care CRP determinations.
G.Koukouvinos, D.Goustouridis, K.Misiakos, S.Kakabakos, I.Raptis, P.Petrou Sens. Act. B 260 282(2018) “Rapid C-reactive protein determination in whole blood with a White Light Reflectance Spectroscopy label-free immunosensor for Point-of-Care applications”
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